Joshi started his career in theatre in Pune. He was noticed through the play Kuryat Sada Tingalam. Along with Anand Abhyankar and Suhasini Deshpande, the show was performed more than 1000 times.
Joshi later on got various roles in Hindi and Marathi films. His 1993 comedy film Sawat Majhi Ladki was quite successful. Joshi played the lead role of a doctor husband of actress Neena Kulkarni and who is engaged in an extra-marital affair with his junior doctor played by Varsha Usgaonkar. For his work in Tu Tithe Mee (1998), Joshi’s work was appreciated. Produced by Smita Talwalkar and directed by Sanjay Surkar, the film was adjudged as the Best Feature Film in Marathi at the 46th National Film Awards. In their official citation, the jury noted Joshi’s and his co-star Suhas Joshi‘s works by quoting “Beautiful performance by Mohan Joshi and Suhas Joshi are the highlights of the film.” He won his only National Film Awards till now for 1999 Marathi film Gharabaher as a Special Mention (Feature Film) “for his wonderfully controlled performance of a corrupt politician.” at 47th National Film Awards. In 2009, he played the lead role of Gadge Maharaj, a social reformer and saint from Maharashtra, in the film Debu.
Joshi also played various character and side roles in Hindi films and is known for his portrayal of negative roles. For the role of Tirpat Singh in the Hindi film Mrityudand (1997), Joshi received the Screen Award for Best Villain. His villainous role of Sadhu Yadav in the 2003 Hindi film Gangaajal directed by Prakash Jha was appreciated. This socio-political film was based on the Bhagalpur blindings that happened in 1980. He has also worked in Bhojpuri films.
Joshi stepped in the Hindi television industry through the show Jamuniya in 2010 that aired on Imagine TV.
Joshi was president of the Akhil Bharatiya Marathi Natya Parishad from 2003 to 2011 and then reelected in 2013 to at present. .
He married to Ms.Jyoti. .
|Abhi To Mein Jawan Hu||Sony|
|2009||Agnihotra||Chintamani Agnihotri||Marathi||Star Pravah|
|2010-2011||Dhoondh Legi Manzil Humein||Nityanand Tiwari||Hindi||STAR One|
|2012||Eka Lagnachi Dusri Goshta||Shripad Kale||Marathi||Zee Marathi|
|2012–2014||Gunda Purush Dev||Mahendra Deshmukh (Anna)||Marathi||ETV Marathi|
|2013–2014||Eka Laganachi Tisari Gost||Dattaram Bhau||Marathi||Zee Marathi|
|03-August-2015||Chala Hawa Yeu Dya||Guest||Marathi||Zee Marathi|
|28-March-2016||Kahe Diya Pardes||Madhusudhan Sawant||Marathi||Zee Marathi|